Artificial morphogen-mediated differentiation in synthetic protocells

Tian, L., Li, M., Patil, A. J., Drinkwater, B. W., & Mann, S. (2019). Artificial morphogen-mediated differentiation in synthetic protocells. Nature Communications, 10(1), 1–13.

https://doi.org/10.1038/s41467-019-11316-4

Abstract

The design and assembly of artificial protocell consortia displaying dynamical behaviours and systems-based properties are emerging challenges in bottom-up synthetic biology. Cellular processes such as morphogenesis and differentiation rely in part on reaction-diffusion gradients, and the ability to mimic rudimentary aspects of these non-equilibrium processes in communities of artificial cells could provide a step to life-like systems capable of complex spatiotemporal transformations. Here we expose acoustically formed arrays of initially identical coacervate micro-droplets to uni-directional or counter-directional reaction-diffusion gradients of artificial morphogens to induce morphological differentiation and spatial patterning in single populations of model protocells. Dynamic reconfiguration of the droplets in the morphogen gradients produces a diversity of membrane-bounded vesicles that are spontaneously segregated into multimodal populations with differentiated enzyme activities. Our results highlight the opportunities for constructing protocell arrays with graded structure and functionality and provide a step towards the development of artificial cell platforms capable of multiple operations.

In the press

https://www.bristol.ac.uk/wolfson-bioimaging/news/shape-shifting-protocells-hint-at-the-mechanics-of-early-life-.html

https://phys.org/news/2019-07-shifting-protocells-hint-mechanics-early.html

http://www.spacedaily.com/reports/Shape_shifting_protocells_hint_at_the_mechanics_of_early_life_999.html

 

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